Peer Review Request - Decline Confirmation
BMS-EMIDDT-2024-237
Article Title:
TCF7L2 as a target of peripheral artery disease in type 2 diabetes patients: A two-sample Mendelian randomization and bioinformatics study
Abstract:
Objectives: This study examines the causal relationship and potential mechanisms based on analysis of Gene Expression Omnibus (GEO) database and two-sample Mendelian randomization (MR).
Methods: The first part involved a two-sample MR study and comprehensive meta-analysis. Result differences were assessed using inverse variance weighting (IVW). Heterogeneity was examined through Cochrane's Q statistical test and leave-one-out analysis. The potential horizontal pleiotropy effect was assessed utilizing the MR-Egger intercept technique. The second part conducted differential gene analysis (DEGs) and weighted gene co-expression network analysis (WGCNA). Subsequently, we overlapped and consolidated the results from the two parts to obtain the key genes between them.
Results: The MR analysis results suggested a statistically significant correlation between the incidence of PAD and T2D (OR: 1.22, 95% CI: 1.13 to 1.32, p = 3.74e-07). We anticipated the pivotal role of TCF7L2 between PAD and T2D.
Conclusion: T2D is found to be significantly associated with the risk of PAD. Simultaneously, the study deepened our comprehension of the underlying mechanisms in both diseases, proposing TCF7L2 as a promising target.